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After reviewing the blocklist.txt file, I noticed that the CellProfiler features were hardcoded for specific naming conventions for the channels (e.g., DNA, AGP, RNA`).
If someone were to name their channel Hoechst instead of DNA, wouldn't that mean that this file would not work if called?
Would it make more sense to change this default blocklist to avoid the features themselves (e.g., Manders, RWC, and Nuclei Granularity)?
Is there any documentation on why these features were selected as default if you use this operation?
I am hoping more clarity on this file will provide better guidance for processing my datasets.
The text was updated successfully, but these errors were encountered:
After reviewing the blocklist.txt file, I noticed that the CellProfiler features were hardcoded for specific naming conventions for the channels (e.g.,
DNA
, AGP,
RNA`).If someone were to name their channel
Hoechst
instead ofDNA
, wouldn't that mean that this file would not work if called?Would it make more sense to change this default blocklist to avoid the features themselves (e.g., Manders, RWC, and Nuclei Granularity)?
Is there any documentation on why these features were selected as default if you use this operation?
I am hoping more clarity on this file will provide better guidance for processing my datasets.
The text was updated successfully, but these errors were encountered: