diff --git a/dts/arm/nuvoton/npcx/npcx.dtsi b/dts/arm/nuvoton/npcx/npcx.dtsi
index 125ebb656334..2265596a1360 100644
--- a/dts/arm/nuvoton/npcx/npcx.dtsi
+++ b/dts/arm/nuvoton/npcx/npcx.dtsi
@@ -12,6 +12,7 @@
 #include <zephyr/dt-bindings/flash_controller/npcx_fiu_qspi.h>
 #include <zephyr/dt-bindings/gpio/gpio.h>
 #include <zephyr/dt-bindings/i2c/i2c.h>
+#include <zephyr/dt-bindings/i2c/npcx-i2c.h>
 #include <zephyr/dt-bindings/pinctrl/npcx-pinctrl.h>
 #include <zephyr/dt-bindings/pwm/pwm.h>
 #include <zephyr/dt-bindings/sensor/npcx_tach.h>
@@ -549,7 +550,7 @@
 			compatible = "nuvoton,npcx-i2c-port";
 			#address-cells = <1>;
 			#size-cells = <0>;
-			port = <0x00>;
+			port = <NPCX_I2C_CTRL_PORT(0, 0)>;
 			controller = <&i2c_ctrl0>;
 			status = "disabled";
 		};
@@ -558,7 +559,7 @@
 			compatible = "nuvoton,npcx-i2c-port";
 			#address-cells = <1>;
 			#size-cells = <0>;
-			port = <0x10>;
+			port = <NPCX_I2C_CTRL_PORT(1, 0)>;
 			controller = <&i2c_ctrl1>;
 			status = "disabled";
 		};
@@ -567,7 +568,7 @@
 			compatible = "nuvoton,npcx-i2c-port";
 			#address-cells = <1>;
 			#size-cells = <0>;
-			port = <0x20>;
+			port = <NPCX_I2C_CTRL_PORT(2, 0)>;
 			controller = <&i2c_ctrl2>;
 			status = "disabled";
 		};
@@ -576,7 +577,7 @@
 			compatible = "nuvoton,npcx-i2c-port";
 			#address-cells = <1>;
 			#size-cells = <0>;
-			port = <0x30>;
+			port = <NPCX_I2C_CTRL_PORT(3, 0)>;
 			controller = <&i2c_ctrl3>;
 			status = "disabled";
 		};
@@ -585,7 +586,7 @@
 			compatible = "nuvoton,npcx-i2c-port";
 			#address-cells = <1>;
 			#size-cells = <0>;
-			port = <0x41>;
+			port = <NPCX_I2C_CTRL_PORT(4, 1)>;
 			controller = <&i2c_ctrl4>;
 			status = "disabled";
 		};
@@ -594,7 +595,7 @@
 			compatible = "nuvoton,npcx-i2c-port";
 			#address-cells = <1>;
 			#size-cells = <0>;
-			port = <0x50>;
+			port = <NPCX_I2C_CTRL_PORT(5, 0)>;
 			controller = <&i2c_ctrl5>;
 			status = "disabled";
 		};
@@ -603,7 +604,7 @@
 			compatible = "nuvoton,npcx-i2c-port";
 			#address-cells = <1>;
 			#size-cells = <0>;
-			port = <0x51>;
+			port = <NPCX_I2C_CTRL_PORT(5, 1)>;
 			controller = <&i2c_ctrl5>;
 			status = "disabled";
 		};
@@ -612,7 +613,7 @@
 			compatible = "nuvoton,npcx-i2c-port";
 			#address-cells = <1>;
 			#size-cells = <0>;
-			port = <0x60>;
+			port = <NPCX_I2C_CTRL_PORT(6, 0)>;
 			controller = <&i2c_ctrl6>;
 			status = "disabled";
 		};
@@ -621,7 +622,7 @@
 			compatible = "nuvoton,npcx-i2c-port";
 			#address-cells = <1>;
 			#size-cells = <0>;
-			port = <0x61>;
+			port = <NPCX_I2C_CTRL_PORT(6, 1)>;
 			controller = <&i2c_ctrl6>;
 			status = "disabled";
 		};
@@ -630,7 +631,7 @@
 			compatible = "nuvoton,npcx-i2c-port";
 			#address-cells = <1>;
 			#size-cells = <0>;
-			port = <0x70>;
+			port = <NPCX_I2C_CTRL_PORT(7, 0)>;
 			controller = <&i2c_ctrl7>;
 			status = "disabled";
 		};
diff --git a/dts/arm/nuvoton/npcx/npcx4.dtsi b/dts/arm/nuvoton/npcx/npcx4.dtsi
index 6bc44daae1d2..130e752daa76 100644
--- a/dts/arm/nuvoton/npcx/npcx4.dtsi
+++ b/dts/arm/nuvoton/npcx/npcx4.dtsi
@@ -449,7 +449,7 @@
 			compatible = "nuvoton,npcx-i2c-port";
 			#address-cells = <1>;
 			#size-cells = <0>;
-			port = <0x40>;
+			port = <NPCX_I2C_CTRL_PORT(4, 0)>;
 			controller = <&i2c_ctrl4>;
 			status = "disabled";
 		};
@@ -458,7 +458,7 @@
 			compatible = "nuvoton,npcx-i2c-port";
 			#address-cells = <1>;
 			#size-cells = <0>;
-			port = <0x71>;
+			port = <NPCX_I2C_CTRL_PORT(7, 1)>;
 			controller = <&i2c_ctrl7>;
 			status = "disabled";
 		};
diff --git a/dts/bindings/i2c/nuvoton,npcx-i2c-port.yaml b/dts/bindings/i2c/nuvoton,npcx-i2c-port.yaml
index 8f5f288705e8..76304f57374b 100644
--- a/dts/bindings/i2c/nuvoton,npcx-i2c-port.yaml
+++ b/dts/bindings/i2c/nuvoton,npcx-i2c-port.yaml
@@ -11,7 +11,13 @@ properties:
   port:
     type: int
     required: true
-    description: index of i2c port
+    description: |
+      Index of i2c port
+      This property is used to differentiate between multiple I2C ports controlled by the same
+      or different I2C controllers.
+      Bit[7:4] module id (controller).
+      Bit[3:0] port id.
+      Use the macro NPCX_I2C_CTRL_PORT(ctrl, port) to set this property.
 
   controller:
     type: phandle
diff --git a/include/zephyr/dt-bindings/i2c/npcx-i2c.h b/include/zephyr/dt-bindings/i2c/npcx-i2c.h
new file mode 100644
index 000000000000..fc46fbf55fd0
--- /dev/null
+++ b/include/zephyr/dt-bindings/i2c/npcx-i2c.h
@@ -0,0 +1,12 @@
+/*
+ * Copyright (c) 2024 Nuvoton Technology Corporation.
+ *
+ * SPDX-License-Identifier: Apache-2.0
+ */
+#ifndef ZEPHYR_INCLUDE_DT_BINDINGS_I2C_NPCX_H_
+#define ZEPHYR_INCLUDE_DT_BINDINGS_I2C_NPCX_H_
+
+
+#define NPCX_I2C_CTRL_PORT(ctrl, port) (((ctrl & 0xf) << 4) | (port & 0xf))
+
+#endif	/* ZEPHYR_INCLUDE_DT_BINDINGS_I2C_NPCX_H_ */